Starting observations with 4x magnification is not a universal requirement, but it can be beneficial in certain contexts, particularly in microscopy. Here are a few reasons why starting with 4x magnification may be important or advantageous:
Field of view: Lower magnifications provide a wider field of view, allowing you to observe a larger area. This is particularly useful when you're trying to locate a specific object or get a general overview of a sample. Starting with 4x magnification helps you establish spatial awareness and locate areas of interest before zooming in further.
Depth of field: Higher magnifications often have a shallower depth of field, meaning that only a narrow plane will be in focus at a time. By starting with 4x magnification, you can initially get a broader view of the sample, which may help you identify the most suitable region to focus on later.
Sample navigation: When dealing with complex or dense samples, starting with a lower magnification allows you to navigate through the specimen more easily. It helps you identify different structures, regions, or features, which can guide your subsequent observations at higher magnifications.
Context and orientation: Lower magnifications provide a better understanding of the overall context and orientation of the sample. This can be particularly important in research, where you need to document the location and relationship of various structures or objects within the sample.
Preventing damage: Starting with higher magnifications immediately may increase the risk of accidentally damaging or disturbing the sample. By beginning with a lower magnification, you can ensure you're in the correct position and avoid unintentional contact or disruption.