Biological Microscope

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1. Rich Experience
By several years development, we have become a professional supplier for different kinds of microscopes, telescopes, binoculars, magnifiers, spotting scopes, riflescopes and accessories.

2. Reliable Product Quality
We practise ISO9001:2015 quality standard. Our all microscopes have passed CE certificate, some items has ROHS certificate. The astronomical telescope and binoculars also passed EN71 certificate.

3. Professional Technical Team
Our experienced workers and strict inspection system guarantee that goods will be with best quality before shipment. R&D team continuously optimizes available models and designs new models to keep our products' competition in the market.

4. Customizable Services
We can supply OEM and ODM service, additionally we can offer other service, such as logo printing, color box design, shipment arrangement, etc.

What is Biological Microscope?

A Biological Microscope also referred to as a Compound Microscope allows the researcher to study microorganisms, cells, crystalline structures, molecular structures, and tissue sample reading to examine microscopic samples of specimens. Biological microscopes are employed by researchers and scientists, and lab technicians to scrutinize the reading of transparent objects to thick cultures. In a Biology Lab, these microscopes provide greater resolutions of biological cells and tissues, with the ability to explore and zoom much higher magnifications.

Advantages of Biological Microscope

High magnification
Biological microscopes can magnify objects up to 3,000 times their original size, making them invaluable for studying minute details of cells, microorganisms, and other small objects.

Wide field of view
Biological microscopes typically have a wider field of view than simple microscopes, allowing for the observation of a larger area of the specimen at once.

Versatility

Biological microscopes can be used to study a wide variety of specimens, including living cells, bacteria, viruses, and other microscopic organisms.

Ability to view live specimens

Biological microscopes can be used to observe live specimens, providing valuable insights into cellular processes and organism behavior.

Relatively affordable

Biological microscopes are relatively affordable compared to other types of microscopes, such as electron microscopes.

Application of Biological Microscope

Cellular, Pathological, and Microbial Research

The biological microscope is the most common type of biological microscope used to observe biological specimens at high magnification. Phase contrast microscope is a special type of biological microscope that differs from the ordinary brightfield microscope. It requires phase contrast objective lenses and a condenser to alter the phase of light passing through the specimen, thereby enhancing contrast. It is mainly used for observing blood cells, live cells, and unstained transparent specimens.

Mineral and Rock Analysis

The polarizing microscope is another common type of biological microscope, featuring two crucial polarizing devices – the polarizer and the analyzer. The polarizer is positioned between the light source and the specimen, converting natural light into polarized light. The analyzer is located between the specimen and the eyepiece and is used to analyze the vibration direction of light passing through the specimen. The polarizing microscope is commonly used for the analysis of the structure and properties of minerals and rocks.

Metal Analysis

The metallographic microscope, or metallurgical microscope, is also a type of biological microscope primarily used for observing metals, alloy materials, semiconductor testing, ceramics, rocks, and other opaque materials. Therefore, metallographic microscopes typically employ high-intensity reflected light, which is directed through the objective lens onto the specimen, producing high-contrast images of opaque specimens. They can also simultaneously utilize both transmitted and reflected light.

Components of Biological Microscope

Arm

It is in the back of the microscope and supports the objectives and ocular. Also, it is the part that we use to carry or lift it.

Course Focusing Knob

We use it to adjust the position of objective lenses. Also, this should be done keeping in mind that the objective should not hit the slide. In addition, it should be stopped when the object is completely visible through the ocular.

Fine Focusing Knob

We use it to bring the specimen in perfect focus once the specimen is visible through the course-focusing knob. Also, focus slowly to avoid contact between the objective and the specimen.

Oil immersion Lens

This is a 100x (100 times) objective lens. Also, this lens is small in order to attain high resolution and magnification. Furthermore, due to its size, it is important for the lens to get as much light as possible.

Base

It’s the bottom of the scope. In addition, it houses the light source and the back section of base acts as a handle to carry the scope.

Illuminator

It is the light source of the microscope.

Numerical Aperture or Objective lens

It is found in a compound scope and is the lens that is closest to the specimen.

Ocular Lens

This is the lens closest to the viewer in a compound light microscope.

How a Biological Microscope Works?

Optical Components
A biological microscope works by using a series of optical components to magnify an object. The first component is the objective lens, which is located near the object being viewed. The objective lens collects and focuses light from the object onto the second component, the eyepiece lens. The eyepiece lens then magnifies the image formed by the objective lens and projects it onto the retina of the observer's eye. In addition to the objective and eyepiece lenses, a biological microscope also includes a light source, typically an LED or halogen bulb, to illuminate the object being viewed. The light passes through a condenser lens, which focuses the light onto the object and improves the resolution of the image.

Magnification and Resolution
Magnification and Resolution are the two key principles that govern how a biological microscope works. Magnification refers to the ability of the microscope to enlarge an object, while resolution refers to the ability to distinguish between two closely spaced objects. In a biological microscope, light passes through the specimen and is then magnified by two lenses: the objective lens and the eyepiece lens. The objective lens is located close to the specimen and produces a magnified image, which is then further magnified by the eyepiece lens. The total magnification is the product of the magnification of the objective lens and the eyepiece lens.

Illumination
Illumination is a crucial component of how a biological microscope works. The microscope uses a light source to illuminate the specimen being observed. The light passes through the condenser lens, which focuses the light onto the specimen. The specimen then reflects or absorbs some of the light, which is then magnified by the objective lens and the eyepiece lens. In recent years, advancements in illumination technology have greatly improved the capabilities of biological microscopes. LED lights have replaced traditional light sources, providing brighter and more consistent illumination. Additionally, fluorescence microscopy has become a popular technique for observing specific structures within a specimen.

Focusing Mechanisms
One of the key components of a biological microscope is the focusing mechanism. This mechanism allows the user to adjust the focus of the microscope to obtain a clear image of the specimen being observed. There are two main types of focusing mechanisms used in biological microscopes: coarse focus and fine focus. Coarse focus is used to make large adjustments to the focus of the microscope. This is typically done by moving the stage up or down using a coarse focus knob. Fine focus, on the other hand, is used to make small adjustments to the focus of the microscope. This is typically done by moving the stage up or down using a fine focus knob.

How Does a Biological Microscope Magnify an Object?

1. Objective lens magnifies the object
A biological microscope is an optical instrument that uses two lenses to magnify an object. The two lenses are the objective lens and the eyepiece lens. The objective lens is located near the object being viewed and is responsible for magnifying the object. The eyepiece lens is located near the viewer's eye and is responsible for further magnifying the image produced by the objective lens. The objective lens magnifies the object by bending the light that passes through it. The amount of bending that occurs depends on the shape of the lens and the refractive index of the material it is made of. The objective lens is designed to have a short focal length, which means that it can focus light from a nearby object onto a small area of the microscope's stage.

2. Eyepiece lens further magnifies the image
A biological microscope is an optical instrument that uses two lenses to magnify an object. The objective lens is located near the specimen and produces a real, inverted image. The eyepiece lens is located near the observer's eye and further magnifies the image produced by the objective lens. The combination of these two lenses produces a highly magnified image of the specimen. The objective lens is the primary magnifying lens in a biological microscope. It is a high-powered lens that is designed to produce a real, inverted image of the specimen. The objective lens is located close to the specimen and collects light from it. The light is then focused by the lens to produce a magnified image of the specimen.

3. Total magnification is the product of both lenses
A biological microscope is an optical instrument that uses two lenses to magnify an object. The lenses are the objective lens and the eyepiece lens. The objective lens is located near the object being viewed and produces a real, inverted image of the object. The eyepiece lens is located near the viewer's eye and magnifies the image produced by the objective lens. The magnification of a biological microscope is determined by the focal lengths of the objective and eyepiece lenses. The magnification of the objective lens is fixed and is usually printed on the lens. The magnification of the eyepiece lens can be adjusted by changing the distance between the lens and the viewer's eye.

4. Resolution is limited by wavelength of light
A biological microscope magnifies an object by using two lenses, the objective lens and the eyepiece lens. The objective lens is located near the object being viewed and produces a magnified image of the object. This image is then further magnified by the eyepiece lens, which is located near the viewer's eye. The combination of these two lenses produces a greatly magnified image of the object. The magnification power of a biological microscope is determined by the focal length of the objective lens and the eyepiece lens. The greater the focal length of these lenses, the greater the magnification power of the microscope.

Difference between Biological and Electron Microscope

The difference between a Biological and electron microscope is lited below in the table.

Sr.No	Biological Microscope	Electron Microscope
1.	A light beam is used to illuminate the item.	An electron beam is used.to illuminate the item.
2.	It makes use of glass lenses.	It makes use of electromagnetic devices.
3.	There is no need for an internal vacuum.	An internal vacuum is required.
4.	It magnifies the item about 2000 times.	It magnifies the item about 200,000 times.

Precautions While Using Biological Microscope

● Never put your fingers near the glass portion of the lenses. If you want to clean your lenses, only use special lens paper.
● When not in use, turn off the light source for the microscope. As a result, lamps will last longer and use less energy.
● Using both hands when holding a microscope is recommended. Take hold of the arm with one hand and support it with the other hand by placing it beneath the base.
● Always use a coverslip while doing temporary (wet mount) preparations.
● Remove the slide from the stage and rotate the lowest power objective lens into place before putting the microscope away.

Operating Procedure of Biological Microscope

● The lowest power objective lens (for example, 4x) should be snapped into place by rotating the revolving turret/nosepiece.
● Place the microscope slide on the stage and secure it using the stage clips.
● Turn the focus knob to raise the stage as you look at the objective lens and stage from the side. Without letting the objective hit the coverslip, raise it as high as it will go.
● Adjust the focus knob to focus the image while looking through the eyepiece.
● Adjust the light’s brightness and the condenser’s position for the high amount of light.
● When the sample is in the center of the field of vision, move the microscope slide.
● For the clearest image, adjust the condenser and light intensity after focusing the sample using the focus knob.
● You can switch to the next objective lenses once you obtain a clear image of your sample with the lowest power objective. The sample’s focus and/or the condenser and light intensity may need to be adjusted.
● Make sure the objective lens doesn’t touch the slide!
● After you’re done, lower the stage, click the low power lens into place, and then take out the slide.

How Many Lenses Are in a Biological Microscope?

Objective lenses

A biological microscope is an optical instrument that uses multiple lenses to magnify small objects. The number of lenses in a biological microscope can vary depending on the specific model and design. However, the most common type of lenses found in a biological microscope are the objective lenses. Objective lenses are the primary lenses in a biological microscope that are responsible for gathering light and magnifying the specimen. These lenses are typically located on a rotating nosepiece and can be interchanged to achieve different levels of magnification. The number of objective lenses in a biological microscope can range from two to six, with the most common being four.

Eyepiece lens

A biological microscope is an optical instrument that uses multiple lenses to magnify small objects. The primary purpose of a biological microscope is to provide a detailed view of microscopic specimens, allowing scientists, researchers, and students to study the intricate details of various samples. When it comes to the number of lenses in a biological microscope, there are typically two main lenses involved: the objective lens and the eyepiece lens. The objective lens is located near the specimen and is responsible for gathering light and forming the initial magnified image. The eyepiece lens, on the other hand, is positioned at the top of the microscope and further magnifies the image formed by the objective lens, allowing the viewer to see a highly magnified and detailed image.

Condenser lens

A biological microscope is an optical instrument that uses multiple lenses to magnify small objects. It consists of two main lens systems: the objective lens and the eyepiece lens. The objective lens is located near the specimen and is responsible for gathering light and forming a magnified image. The eyepiece lens, on the other hand, is positioned near the viewer's eye and further magnifies the image formed by the objective lens. In addition to these two primary lenses, a biological microscope may also include a condenser lens. The condenser lens is located beneath the stage and its purpose is to focus and direct light onto the specimen. By doing so, it enhances the illumination and improves the clarity of the image.

Immersion oil lens (optional)

A biological microscope is an optical instrument that uses multiple lenses to magnify small objects. It consists of two lens systems: the objective lens and the eyepiece lens. The objective lens is located near the specimen and is responsible for gathering light and forming a magnified image. The eyepiece lens, on the other hand, is positioned near the observer's eye and further magnifies the image formed by the objective lens. The number of lenses in a biological microscope can vary depending on the design and complexity of the instrument. Typically, biological microscopes have two or more lenses in the objective lens system and one lens in the eyepiece. The objective lens system is composed of multiple lenses that work together to provide high magnification and resolution.

Essential Maintenance Tips for Your Biological Microscope

Cover it up
When not in use, always cover your microscope with a dust cover or a cloth so as to avoid dust and debris from settling on its delicate parts.

Care for Optics
Use a soft lint-free cloth or lens paper to clean the objective and eyepiece lenses regularly. Avoid rough materials that can cause scratches on the lenses. If need be, employ an optical lens cleaning solution.

Cleaning Stage and Condenser
Wipe down the stage and condenser using moistened cloth to get rid of dirt or stains that may have accumulated over time. Internal components should not be exposed to moisture.

Check Light Source
Check if the light source (built-in lamp/outside source) is functioning properly. Change any bulbs that need replacement plus clearing any filters or lenses linked with the illumination system.

Inspect Mechanical Parts
Regularly examine mechanical parts like focusing knobs, stage controls, turret etc., for smooth operations. However, use caution as too much oil will attract dirt and dust thereby overdoing lubrication.

Alignment
This happens more often when microscopes are moved after being transported by checking alignment of optical components in it. Misalignment causes images which are not clearly visible thus distorting them.

Store Properly
Store your microscope in an environment free from dirt and dryness that is away from direct sunlight or extreme temperatures; wet environments should be avoided since they have water vapor leading to damages on both optics and mechanics inside.

Regular Servicing
It might also be helpful to set up regular servicing appointments with a professional technician who can conduct thorough cleaning, alignment adjustment, as well as any necessary repair work.

Handle Gently
Always avoid rough handling or sudden jolts while using these delicate pieces of equipment lest you damage them.

Our Factory

Ningbo Barride Optics Co., Ltd is a manufacture-based company about optical and electronic products. Located in the beautiful port city Ningbo, which has convenient transportation. By several years development, we have become a professional supplier for different kinds of microscopes, telescopes, binoculars, magnifiers, spotting scopes, riflescopes and accessories. At the same time, we also export night vision, rangefinder, compass and other optical products according to clients' requirements.

Our Certifications

We practise ISO9001:2015 quality standard. Our all microscopes have passed CE certificate, some items has ROHS certificate. The astronomical telescope and binoculars also passed EN71 certificate.

Ultimate FAQ Guide to Biological Microscope

Q: How does a biological microscope work?

A: A biological microscope is a type of optical microscope that is primarily designed to observe cells, tissues, and other biological specimens. Multiple objective lenses can be attached, which gives these microscopes a magnification that can range anywhere from 10x - 1,000x or more.

Q: What can you see with a biological microscope?

A: Biologists typically use microscopes to view all types of cells, including plant cells, animal cells, protozoa, algae, fungi, and bacteria.

Q: What factors should you consider before selecting a microscope for use in a biological study?

A: You should consider several factors when purchasing a microscope, especially if you want to choose the right microscope. For example, the microscope's magnification range, the type of sample you are working with, your budget, the microscope's quality, and the purpose for which you need the microscope.

Q: What is the principle of biological microscope?

A: A general biological microscope mainly consists of an objective lens, ocular lens, lens tube, stage, and reflector. An object placed on the stage is magnified through the objective lens. When the target is focused, a magnified image can be observed through the ocular lens.

Q: For what research are biological microscopes used?

A: A Biological Microscope also referred to as a Compound Microscope allows the researcher to study microorganisms, cells, crystalline structures, molecular structures, and tissue sample reading to examine microscopic samples of specimens.

Q: How do I choose a biological microscope?

A: In general, you should consider five main characteristics when choosing a microscope: magnification, observation technique, configuration, number of eyepieces, and type of lighting. The total magnification of the microscope: this is the product of the magnification of the eyepieces and the objective.

Q: How to see bacteria in a microscope?

A: The best way to observe bacteria is by using a compound light microscope. This type of microscope provides the necessary magnification and resolution to visualize bacteria. To ensure accurate observations, it is important to follow proper techniques.

Q: What color is sperm under a microscope?

A: View the slide under 200-400x magnification. Epithelial cells will stain green with red nuclei. Sperm cells will stain red with green tails.

Q: What is the most commonly used microscope in biology?

A: Light microscopes are commonly used in cell biology to observe living or fixed cells and tissues and in medical laboratories for routine examination of biological samples, such as blood smears and tissue sections.

Q: What are the applications of biological microscopes?

A: The most common application of microscopy in microbiology is to observe the morphology or physical properties of microorganisms. Observing the shape, size, and arrangement of cells can provide valuable information for the identification and classification of microorganisms.

Q: How to see lactobacillus under a microscope?

A: The bacteria can be easily seen by preparing a smear or slide of diluted curd and then staining it with safranin or methyl blue. Lactobacillus bacteria can be seen as rod shaped cells.

Q: Can you see parasites under microscope?

A: Protozoan trophozoites, cysts, oocysts, and helminth eggs and larvae may be seen and identified using a wet mount identification technique. To prepare a wet mount, obtain a microscope slide and the stool specimen. Take a small amount of the specimen and place it on a microscope slide.

Q: What can you see with a biological microscope?

A: Biologists typically use microscopes to view all types of cells, including plant cells, animal cells, protozoa, algae, fungi, and bacteria.

Q: How do you prepare a biological specimen for a microscope?

A: The first step is fixation of the cell.
The next step is dehydrating or freezing the specimen.
The next step after processing is embedding.
The next step is sectioning.

Q: Can you see tapeworm eggs under microscope?

A: The redworm eggs are in the pink circle and the tapeworm eggs are in the blue circle. We rarely see tapeworm eggs in a worm egg count but because these are laid in packets in the poo rather than being evenly distributed like redworm and ascarids, it isn't a reliable test for this sort of worm.

Q: What bacteria can be seen with a microscope?

A: Bacillus subtilis: This bacterium is commonly found in soil and the gastrointestinal tract of animals.
Staphylococcus aureus: This bacterium is a common cause of skin infections and is often found in the nasal passages of humans.

Q: What magnification is a biological microscope?

A: Multiple objective lenses can be attached, which gives these microscopes a magnification that can range anywhere from 10x - 1,000x or more.

Q: How to distinguish between bacteria and fungi under a microscope?

A: Upon these agar mediums, bacteria manifest as microscopically tiny oily spot formations. Conversely, fungi manifest as a powdery mat spreading across the entire surface of the agar plate. A primary distinction between bacterial and fungal colonies arises from their origins.

Q: What do pinworms look like under a microscope?

A: Pinworm eggs are asymmetrical, ovoid with a flattened or dimpled side. They measure about 50 x 30 mM while adult worms measure 3-8 mM in length and are about 0.5 mM wide, and white to gray in color. Adult worms are not commonly observed.

Q: How to see coccidia under a microscope?

A: Examine the specimen for worm eggs and Coccidia Oocysts. Start with the lowest magnification (40x) on the microscope and move up to 400x and if you have it, 1000x. You should be able to identify Coccidia Oocysts, Nematode Eggs and Taperworm eggs.

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